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    These studies have gained international recognition; thus, the K-CHIP has been shown to be an appropriate tool for analyzing SNPs associated with diseases in a Korean population.To the best of our knowledge, this study was the first to investigate HTN-related SNPs using the K-CHIP in a Korean population.The amplification products were analyzed in an optimized reaction to amplify fragments between 2 bp in length.A fragmentation step reduced the amplified products to segments of approximately 25–50 bp in length, which were end-labeled using biotinylated nucleotides.The serum fasting glucose level was measured using the hexokinase method with the GLU Kit (Roche, Mannheim, Germany), and the resulting color reaction was monitored with the Hitachi 7600 autoanalyzer (Hitachi, Tokyo, Japan).Serum fasting insulin was measured with an immunoradiometric assay using the Insulin IRMA Kit (DIAsource, Louvain, Belgium), and the resulting color reaction was monitored with an SR-300 system (Stratec, Birkenfeld, Germany).The samples were placed in an ice box that was protected from light within approximately 30 min and then centrifuged (1200 rpm for 20 min at 4 °C) within 3 h to obtain plasma and serum.

    The images were analyzed using the Genotyping Console™ Software (Affymetrix, Santa Clara, CA, USA).Latex bead (30 nm)-conjugated thyroglobulin (17 nm), ferritin (12.2 nm), and catalase (10.4 nm) standards were used to measure the relative band migration rates.The gels were scanned using a GS-800 Calibrated Imaging Densitometer (Bio-Rad Laboratories, Hercules, CA, USA).Since 2014, the Korean Chip (K-CHIP), which includes 833,535 SNPs and uses an oligomer as a probe, has been developed by the Korea Biobank Array project as a low-cost customized chip that is optimized for genetic studies of diseases and complex traits in Koreans (Additional file : Table S1).Since whole-genome sequencing requires very high calculation capacity and cost and commercial chips are designed for Western populations, whose genomic variants differ from Asian populations, the K-CHIP is more suitable for the discovery and identification of Korean population-specific SNPs related to disease occurrence [].

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